Importing PeakNet (Releases 4.3 through 5.2) Method Files

Overview

In order to use Method files from PeakNet (releases 4.3 through 5.2) with Chromeleon, they must first be imported into Chromeleon.

When imported, the PeakNet 5 Method file is converted into a Chromeleon PGM File and/or Quantification Method (QNT Method). Converted PGM Files contain timed events and setup parameters for all modules included in the original PeakNet 5 Method. Converted QNT Files contain component names, retention times, reference peaks, tolerance, calibration options, groups, and level amounts for each detector in the PeakNet 5 Method.

PeakNet 5 Methods from the following modules can be imported: AS50, GP40/GP50, IP20/IP25, AD20, AD25, CD20/CD25, ED40/ED50, IC20/IC25, DX-120, and UI20. For Methods that were created with AI-450 software, only the QNT portion of the Method is converted.

Because of differences in the way that PeakNet 5 and Chromeleon function, some features available in a PeakNet 5 Method file are not imported into Chromeleon. These include:

• Replicate calibration information

• CE Method parameters

• High/low limit values

• Outlier rejection

• Linear weighting options

In addition, Chromeleon allows only a single component table per injection, whereas PeakNet 5 allows one table per detector. When the PeakNet 5 Method contains multiple detectors, the first detector is converted entirely; that is, information about every component and unknown peak is added to the peak table. Then, for the other detector(s), only unique components and unique unknown groups not present in the first detector's data are added to the table. This means that any reference component information for detectors other than the first detector may be lost.

How To

1. In the Browser, select a Datasource folder (not a sequence name).

2. On the File menu, point to Import/Restore, point to PeakNet 5, and click Method Files.

3. In the Import PeakNet 5 Method Files dialog box, browse for the folder that contains the PeakNet 5 Method files to be imported. These files have the .met file name extension.

4. Select one or more of the Method files and click Add. The files appear in the Selected files list.

5. To convert the PeakNet 5 Method into a Chromeleon Program File, select the Program check box. The program file is created in the folder shown next to the Program check box. The default is the datasource folder currently selected in the Browser. To choose a different location, click the Browse button and select a location.

6. To convert the PeakNet 5 Method component table and calibration parameters into a Chromeleon QNT Method, select the QNT check box. The QNT File is created in the folder shown next to the QNT check box. The default is the datasource folder currently selected in the Browser. To choose a different location, click the Browse button and select a location.

7. Select the Inverted Curve Fitting check box to use inverted calibration (concentration or amount is plotted against measured values). Clear the check box for "normal" curve fitting (measured values are plotted against concentration or amount). See Calibrating  Inverting Dependent and Independent Variables for details.

8. Click Import.

The new files have the same name as the PeakNet 5 Method file, but with PN5 appended and with the .pgm and .qnt file name extensions. Once a Method file is converted, the file name is removed from the Selected files list.

 Tip:

If the QNT Method check box is selected, but the PeakNet 5 Method does not contain a detector module, an empty QNT Method file is created.

Also, refer to Importing PeakNet (Releases 4.3 Through 5.2) Data Files.